The Church of Saint Anne in Staré Brno stands as a testament to the region's rich ecclesiastical history. It evolved through several architectural phases from the early 11th century until its demolition at the end of the 18th century. Located within what is now known as the area of the former Starobrněnský klášter, this site began with a rotunda around the turn of the 10th and 11th centuries. This makes it one of Moravia's earliest documented sacred structures. Its significance was further underscored by its continuous use for burials from the 11th century onwards. Over time, the original rotunda gave way to a Romanesque church dedicated to the Virgin Mary in the late 12th century and eventually transitioned into a Gothic church at the end of the 13th century.

The origins of this site are deeply rooted in the early medieval period when Brno was part of the Kingdom of Bohemia under the Přemyslid dynasty. The initial structure, a rotunda, is believed to have been established around the turn of the 10th and 11th centuries, serving as a significant burial site from its inception. This early church is linked to historical figures such as Henry Zdík, who mentioned it in a document dated approximately 1141, highlighting its importance among the churches in the Czech lands at that time. By the late 12th century, this rotunda was replaced by a Romanesque church dedicated to the Virgin Mary, marking an important architectural transition. This structure was later succeeded by a Gothic church towards the end of the 13th century.

Architecturally, the Church of Saint Anne underwent significant transformations throughout its existence. The initial rotunda featured a single apse on its eastern side and had robust foundations made from local materials like sandstone and brick. When replaced by the Romanesque church in the late 12th century, it boasted a rectangular nave and presbytery, with the structure being both plastered and adorned with painted stonework. The final phase saw the construction of a Gothic church that stood until its demolition at the end of the 18th century.

Throughout its history, the site was associated with notable figures such as Lev z Klobouk, who is linked to a forged document from around 1210 claiming he donated the newly founded Church of the Virgin Mary to the Zábrdovice Monastery. This church served as a parish for Staré Brno and surrounding villages until it was replaced by the Gothic structure in the late 13th century.

The site's historical significance is further highlighted by archaeological findings from excavations conducted between 1976 and 1983, which uncovered remnants of all three phases of construction beneath a courtyard of the Starobrněnský klášter. These discoveries provide valuable insights into the architectural evolution and religious importance of this location over several centuries.

Today, while the Church of Saint Anne no longer stands, its historical footprint remains an intriguing site for those interested in medieval architecture and ecclesiastical history. Visitors can explore the area where these significant structures once stood, gaining a deeper appreciation for the region's rich past. The remnants uncovered through archaeological research offer a tangible connection to the early sacred sites of Moravia, making it a worthwhile destination for anyone keen on exploring historical transformations within Brno.