The Church of the Nativity of the Virgin Mary in Domažlice is a significant cultural monument within the town. Recognized for its historical and architectural importance, it was established in the second half of the 13th century. This parish church has undergone various transformations, resulting in its current late Baroque form with additional modifications over time. Its evolution from an early Gothic structure to a three-nave design highlights its rich history and cultural value.

The origins of the Church date back to around 1270 or possibly the 70s of the 13th century, during a period when Bohemia was part of the Holy Roman Empire under King Ottokar II. This era saw significant political and cultural developments, including the spread of Gothic architecture, which influenced the church's initial construction as a two-nave hall with merged nave and chancel. By the 15th century, renovations added a vault and altered its layout to three naves, reflecting architectural trends.

Architecturally, the church features early Gothic elements transitioning into Baroque style through significant modifications between 1751 and 1756. Jiří Záhořík led these alterations, consulting with Kilián Ignác Dientzenhofer. Further changes were made to the façades between 1902 and 1903 under architect Václav Kaura. The design includes a single-nave structure without a distinct chancel area, featuring side aisles covered by four-part cross-ribbed vaults. One of these vaulted areas houses a chapel dedicated to Saint Barbara, while the northern side features a barrel-vaulted chapel of the Virgin Mary.

The church's artistic elements are rich and varied. Two early Gothic portals have been preserved, with one located on the western side being walled up. The nave is covered by three fields of plank vaulting, adorned with paintings depicting scenes from the life of the Virgin Mary. Additionally, a loft in the western section features a fresco of Saint Cecilia by František Julius Luxe. The main altar's illusionistic painted architecture and wall painting in the chapel of Saint Barbara are also attributed to him. Other notable artworks include a statue of Madonna by Munich sculptor F. N. Rietzler from 1876, and a retabulum originally placed in the chapel of Saint Barbara, crafted in 1744 by Prague silversmith A. Karer.

The church's interior is further enhanced by two side altars from the latter third of the 18th century, adorned with lattice and rocaille ornaments featuring sculptures of Saints Peter and Paul, Saint Christopher, and Saint James. Opposite these are an altar dedicated to Saint Anne with a sculpture of the patron saint by František Ringelhahn, and another altar depicting Piety from the late third quarter of the 18th century. The chapel of Saint Barbara houses a neo-Gothic altar with a statue of Saint Wenceslas from the 19th century, while the chapel of Our Lady of the Rosary contains a portal altar from the mid-17th century and an 18th-century baptismal font.

An intriguing feature of the church is its bell tower located in the southwest corner. Its age remains uncertain, with possibilities ranging from the 13th to the 15th centuries. Originally separated from the church by a narrow alley, it was constructed incrementally, reaching its current height by 1545.

Today, the Church of the Nativity of the Virgin Mary is accessible to visitors and stands as a testament to the architectural and cultural heritage of Domažlice. Its historical significance, combined with its artistic treasures, makes it a worthwhile destination for those interested in exploring the rich tapestry of Czech history and architecture. Visitors are invited to appreciate this remarkable monument, which continues to be an integral part of the town's cultural landscape.